Abstract A fundamental challenge for employees in the office environment is the difficulty of being physically active. The long-term effect of physical inactivity can lower work effectiveness and cause health problems. The non-substantial indication from recent research suggests that persuasive techniques can create a significant impact on motivating people. This study investigates the overall influence of using a persuasive application in promoting physical activity in the workplace, such as office environment. To motivate individuals for healthier behaviour, we implemented and tested an application incorporating Self Determination Theory (SDT). We conducted an eight week long usability evaluation of the application, using the UTAUT model. The questionnaires were based on the factors: Performance Expectancy, Effort Expectancy, Social Influence, Facilitating Conditions, Behavioural Intention, and Use Behaviour. We found that our persuasive application was satisfactory to motivate users for physical activity promotion.