Emotional intelligence and risk taking behaviour are considered as significant factors through which people engage in organizations and in daily life. This dissertation formulates the linkage between emotional intelligence, positive affect, negative affect and risk taking behavior. The underlying principle of this study was to develop a sense of relationship between emotional intelligence, positive affect, negative affect, and the conduct of risk taking behavior amongst telecom companies providing the call center services in Pakistan. Afterwards mediating affect of positive and negative affect was checked in the proposed emotional intelligence-risk behavior relationship. Current study was conducted in three big cities of Pakistan (Islamabad, Lahore and Rawalpindi). The technique of convenience sampling was used to collect the data through questionnaires from call center agents. The research design was cross-sectional, and total of 202 finalized and usable responses were used as sample size. In time one 300 questionnaires were used as sample size, and similarly 300 questionnaires were distributed in second time for data collection. Correlation, regression, and descriptive statistics were used to analyze the data. All hypotheses were accepted and it was proved that positive and negative affect mediate the emotional intelligence to risk behavior relationship. Moreover, current research highlights the areas where future research can be done in different context by involving several other constructs with risk taking behavior.